Optimizing Your Research With CRL'S Instrument Services

SEMINAR ROOM, ARC AND CENTAL RESEARCH LAB (CRL), January 7 2025

Optimizing Your Research with CRL's Instrument Services

The Central Research Laboratory (CRL) is committed to empowering researchers with state-of-the-art instrumentation and professional support. Our services are designed to enhance the efficiency and reliability of your experiments, ensuring high-quality outcomes every time.

Highlighted Instrument Services:    

Thermo Multiskan SkyHigh

Purpose: The Multiskan SkyHigh is a microplate spectrophotometer designed for absorbance measurements. It is commonly used for applications requiring high-precision detection of light absorbance in microplate wells.

Applications:    

1. Nucleic Acid Quantification:
   • Measurement of DNA, RNA, and oligonucleotide concentrations for downstream applications.
   • UV/VIS spectroscopy for purity analysis (e.g., A260/A280 ratios).
2. Protein Quantification:
   • Compatible with Bradford, Lowry, and BCA assays to determine protein concentrations.
3. Enzyme Kinetics:
   • Study enzyme activity and reaction rates in a time-resolved manner.
4. Cell-Based Assays:
   • Analyze cellular responses, including proliferation and cytotoxicity (e.g., MTT or XTT assays).
5. ELISA (Enzyme-Linked Immunosorbent Assay):
   • Detect and quantify biomolecules like antibodies or antigens.
6. Spectral Scanning:
   • Full-spectrum analysis for broader wavelength detection and optimization.

Wellwash Versa

Key Features:

Compatibility with a wide range of microplate formats (6 to 48- well, 96- and 384-well plates).

Multiple wavelength detection for flexible applications. (190nm to 1000nm)

User-friendly software for data analysis. (Skanit Software)

Purpose:

The Wellwash Versa is an automated microplate washer designed to simplify and standardize the washing steps in microplate-based assays, ensuring reproducibility and precision.

Applications:  

1. ELISA Washing:
   • Removal of unbound reagents during ELISA workflows to improve assay precision.
2. Cell Washing:
   • Gentle washing of adherent or suspension cells in microplates to maintain sample integrity.
3. Bead-Based Assays:
   • Washing magnetic or polystyrene beads in immunoassays or multiplex assays.
4. Cytokine and Protein Arrays:
   • Ensure thorough washing to reduce background noise and enhance signal clarity.

Key Features:

Flexible washing settings for different plate formats (96- and 384-well plates).

Adjustable wash parameters like dispense speed and aspiration strength for delicate samples.

Automated protocols to save time and reduce human error.

Comparison and Complementary Use:

• Multiskan SkyHigh measures absorbance, providing quantitative assay results.
• Wellwash Versa ensures clean and consistent plate washing, which is critical for reducing background noise in absorbance readings.

For inquiries and bookings:

Visit our website https://research.amdi.usm.my/ipptcrl or contact email to crlippt_services@usm.my