Optimizing Your Research with CRL's Instrument Services

Optimizing Your Research with CRL's Instrument Services

The Central Research Laboratory (CRL) is committed to empowering researchers with state-of-the-art instrumentation and professional support. Our services are designed to enhance the efficiency and reliability of your experiments, ensuring high-quality outcomes every time.

Highlighted Instrument Services:

Thermo Multiskan SkyHigh

Purpose:

The Multiskan SkyHigh is a microplate spectrophotometer designed for absorbance measurements. It is commonly used for applications requiring high-precision detection of light absorbance in microplate wells.

Applications:

1.       Nucleic Acid Quantification:

·      Measurement of DNA, RNA, and oligonucleotide concentrations for downstream applications.

·      UV/VIS spectroscopy for purity analysis (e.g., A260/A280 ratios).

2.       Protein Quantification:

·      Compatible with Bradford, Lowry, and BCA assays to determine protein concentrations.

3.       Enzyme Kinetics:

·      Study enzyme activity and reaction rates in a time-resolved manner.

4.       Cell-Based Assays:

·      Analyze cellular responses, including proliferation and cytotoxicity (e.g., MTT or XTT assays).

5.       ELISA (Enzyme-Linked Immunosorbent Assay):

·      Detect and quantify biomolecules like antibodies or antigens.

6.       Spectral Scanning:

·      Full-spectrum analysis for broader wavelength detection and optimization.

Wellwash Versa

Key Features:

Compatibility with a wide range of microplate formats (6 to 48- well, 96- and 384-well plates).

Multiple wavelength detection for flexible applications. (190nm to 1000nm)

User-friendly software for data analysis. (Skanit Software)

Purpose:

The Wellwash Versa is an automated microplate washer designed to simplify and standardize the washing steps in microplate-based assays, ensuring reproducibility and precision.

Applications:

1.       ELISA Washing:

·      Removal of unbound reagents during ELISA workflows to improve assay precision.

2.       Cell Washing:

·      Gentle washing of adherent or suspension cells in microplates to maintain sample integrity.

3.       Bead-Based Assays:

·      Washing magnetic or polystyrene beads in immunoassays or multiplex assays.

4.       Cytokine and Protein Arrays:

·      Ensure thorough washing to reduce background noise and enhance signal clarity.

Key Features:

Flexible washing settings for different plate formats (96- and 384-well plates).

Adjustable wash parameters like dispense speed and aspiration strength for delicate samples.

Automated protocols to save time and reduce human error.

Comparison and Complementary Use:

·      Multiskan SkyHigh measures absorbance, providing quantitative assay results.

·      Wellwash Versa ensures clean and consistent plate washing, which is critical for reducing background noise in absorbance readings.

For inquiries and bookings:
Visit our website https://research.amdi.usm.my/ipptcrl or contact email to crlippt_services@usm.my